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Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody at 1:1000.
Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody at 1:1000.
PCSK9 Antibody (RB18880) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody at 1:1000.

PCSK9 Antibody

CATALOG NUMBER: 62-832

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-508 amino acids from the C-terminal region of human PCSK9.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
74 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCSK9
Additional Names:
Proprotein convertase subtilisin/kexin type 9, 3421-, Neural apoptosis-regulated convertase 1, NARC-1, Proprotein convertase 9, PC9, Subtilisin/kexin-like protease PC9, PCSK9, NARC1
Protein Accession Number:
Q8NBP7
Protein Gi Number:
317373487
Ncbi Gene Id Number:
255738
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Background Reference 1:
Abifadel,M., Rabes,J.P. Hum. Mutat. 30 (7), E682-E691 (2009)
Background Reference 2:
McNutt,M.C., Kwon,H.J. J. Biol. Chem. 284 (16), 10561-10570 (2009)
Background Reference 3:
Shioji,K., Mannami,T. J. Hum. Genet. 49 (2), 109-114 (2004)
Background Reference 4:
Abifadel,M., Varret,M. Nat. Genet. 34 (2), 154-156 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-832

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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