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Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.
Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 μg/ml.<br><br>Green: PD-1 Antibody (4067) <br> Blue: DAPI staining
Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 μg/ml.
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Trial Size

PD-1 Antibody

CATALOG NUMBER: 4067

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1.
The immunogen is located within amino acids 120 - 170 of PD-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 32 kDa
Observed: 43 kDa
Purification:
PD-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PD-1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDCD1
Additional Names:
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1
Protein Accession Number:
Q15116
Protein Gi Number:
145559515
Ncbi Gene Id Number:
5133
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
Background Reference 1:
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Background Reference 2:
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
Background Reference 3:
Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
Background Reference 4:
Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity1999; 11:141-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4067

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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