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A sandwich ELISA was performed using the anti-PD-1 mAb RF16006 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-1 mAb RF16003-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
A sandwich ELISA was performed using the anti-PD-1 mAb RF16006 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-1 mAb RF16003-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
Trial Size

PD1 Antibody [7H6] (biotin)

CATALOG NUMBER: RF16003-biotin

Clonality:
Monoclonal
Tested Applications:
ELISA
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
PD-1 antibody (biotin) was raised against the extracellular domain of human PD-1.
Conjugate:
Biotin
Tested Applications:
ELISA
Application Note:
PD1 antibody can be used for as detection antibody in Sandwich Elisa at 1 μg/mL.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 3:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 4:
Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 5:
Cat. No. 10-001 - Human Breast Tissue Slide
Positive Control 6:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
PD-1 Antibody (biotin) is supplied as protein A purified IgG1.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PD-1 Antibody (biotin) is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PD-1 Antibody (biotin) can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDCD1
Additional Names:
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
Protein Accession Number:
NP_005009
Protein Gi Number:
145559515
Ncbi Gene Id Number:
5133
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Background Reference 1:
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Background Reference 2:
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
Background Reference 3:
Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
Background Reference 4:
Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

RF16003-biotin

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $109.00 through $452.00

Datasheet
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