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IHC testing of FFPE human tonsil with PD1 antibody. Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
IHC testing of FFPE human tonsil with PD1 antibody. Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

PDCD1 / PD-1 Antibody

CATALOG NUMBER: 33-741

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A full-length human protein was used as the immunogen for the PD1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT
Immunofluorescence: 1-2 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
Titering of the PD1 antibody may be required for optimal performance.
Purification:
Protein A affinity chromatography
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PDCD1
Additional Names:
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
Ncbi Gene Id Number:
5133
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
PDCD1 (Programmed cell death-1 protein), also called PD1 and CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD1 is expressed by few B-cells, so may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-741

List Size:
100 ug

List Price:

$626.00

Datasheet
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