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Western blot analysis of extracts of various cell lines, using PDCD4 antibody (18-641).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of various cell lines, using PDCD4 antibody (18-641).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PDCD4 Antibody

CATALOG NUMBER: 18-641

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human PDCD4
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, IP, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IP: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
MCF7
Predicted Molecular Weight:
Observed: 57kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PDCD4
Additional Names:
PDCD4 Antibody: H731, H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein
Ncbi Gene Id Number:
27250
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-641

List Size:
100 uL

List Price:

$540.00

Datasheet
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