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Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL.
Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 μg/mL.
Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1μg/mL.
Trial Size

PDCD4 Antibody

CATALOG NUMBER: 3975

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
PDCD4 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4.
The immunogen is located within the last 50 amino acids of PDCD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
PDCD4 antibody can be used for detection of PDCD4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
PDCD4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PDCD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PDCD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDCD4
Additional Names:
PDCD4 Antibody: H731, H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein
Protein Accession Number:
NP_663314
Protein Gi Number:
21735598
Ncbi Gene Id Number:
27250
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDCD4 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
Background Reference 1:
Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
Background Reference 2:
Shibihara K, Asano M, Ishida Y, et al. Isolation of a novel mouse gene MA-3 that is induced upon programmed cell death. Gene 1995; 166:297-301.
Background Reference 3:
Goke R, Barth P, Schmidt A, et al. Programmed cell death 4 suppresses CDK1/cdc2 via induction of p21 (Waf1/Cip1). Am. J. Physiol. Cell Physiol. 2004; 287:C1541-6.
Background Reference 4:
Yang HS, Jansen AP, Nair R, et al. The transformation suppressor Pdcd4 is a novel translation initiation factor 4A binding protein that inhibits translation. Mol. Cell Biol. 2003; 23:26-37.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3975

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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