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43-062 (1ug/ml) staining of nuclear NIH3T3 (A) and nuclear HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-062 (1ug/ml) staining of nuclear NIH3T3 (A) and nuclear HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PDCD4 Antibody

CATALOG NUMBER: 43-062

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DSYKGTVDCVQAR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell lines NIH3T3 and HeLa (calculated MW of 51.7kDa according to NP_055271.2). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
Positive Control 1:
Nuclear HeLa Lysate
Predicted Molecular Weight:
Approx 55 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDCD4
Additional Names:
PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), H731, MGC33046, MGC33047, OTTHUMP00000020483, neoplastic transformation inhibitor protein, nuclear antigen H731, programmed cell death protein 4, protein 197/15a
Protein Accession Number:
NP_055271.2,NP_663314.1,NP_001186421.1
Protein Gi Number:
21735596
Ncbi Gene Id Number:
27250

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-062

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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