Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of PDCL in K562 cell lysate with PDCL antibody at 1 μg/ml.
Western blot analysis of PDCL in K562 cell lysate with PDCL antibody at 1 μg/ml.
Trial Size

PDCL Antibody

CATALOG NUMBER: 7871

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
PDCL antibody was raised against a 16 amino acid peptide near the amino terminus of human PDCL.
The immunogen is located within the first 50 amino acids of PDCL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PDCL antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
PDCL antibody is human, mouse and rat reactive. At least two isoforms of PDCL are known to exist. PDCL antibody is predicted to not cross-react with other members of the PDCL protein family.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 33 kDa
Observed: 35 kDa
Purification:
PDCL antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PDCL antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PDCL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PDCL
Additional Names:
PDCL Antibody: PhLP, PHLOP1, PhLP1, Phosducin-like protein, PHLP
Protein Accession Number:
NP_005379
Protein Gi Number:
88758594
Ncbi Gene Id Number:
5082
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL is widely expressed in normal human tissues and may participate directly in the regulation of calcium-evoked exocytosis in adrenal medullary chromaffin cells (3). It is an ethanol-responsive modulator of heterotrimeric G proteins (4).
Background Reference 1:
Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5.
Background Reference 2:
Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30.
Background Reference 3:
Gensse M, Vitale N, Chasserot-Golaz S, et al. Regulation of exocytosis in chromaffin cells by phosducin-like protein, a protein interacting with G protein betagamma-subunits. FEBS Lett. 2000; 480:184-8.
Background Reference 4:
Barhite S, Thibault C, Miles MF, et al. Phosducin-like protein (PhLP), a regulator of G beta gamma function, interacts with the proteasomal protein SUG1. Biochim. Biophys. Acta 1998; 1402: 95-101.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7871

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369