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46-150 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-150 (10ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-150 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PDE11A Antibody

CATALOG NUMBER: 46-150

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NSFKESMEKSSYSD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 60-65kDa band observed in Human Skeletal Muscle and Human Prostate lysates (calculated MW of 65.7kDa according to NP_001070826.1). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise all four reported isoforms (NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1).
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDE11A
Additional Names:
PDE11A, phosphodiesterase 11A , FLJ23693, MGC133355, MGC133356, PPNAD2 , cAMP and cGMP cyclic nucleotide phosphodiesterase 11A, cyclic nucleotide phosphodiesterase 11A, dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
Protein Accession Number:
NP_058649.3,NP_001070665.1,NP_001070826.1,NP_001070664.1
Protein Gi Number:
116536085
Ncbi Gene Id Number:
50940
Background Reference 1:
Horvath A, Boikos S, Giatzakis C, Robinson-White A, Groussin L, Griffin KJ, Stein E, Levine E, Delimpasi G, Hsiao HP, Keil M, Heyerdahl S, Matyakhina L, Libe R, Fratticci A, Kirschner LS, Cramer K, Gaillard RC, Bertagna X, Carney JA, Bertherat J, Bossis I, Stratakis CA. A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia. Nat Genet. 2006 Jul;38(7):794-800. Epub 2006 Jun 11.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-150

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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