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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of PDE3B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

PDE3B Antibody

CATALOG NUMBER: 56-179

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDE3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-427 amino acids from the Central region of human PDE3B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
124 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDE3B
Additional Names:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B, CGIPDE1, CGIP1, Cyclic GMP-inhibited phosphodiesterase B, CGI-PDE B, PDE3B
Protein Accession Number:
Q13370
Protein Gi Number:
143811435
Ncbi Gene Id Number:
5140
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes; PDE3B may play a role in fat metabolism.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Yamano, Y., et al. Int. J. Cancer 126(2):437-449(2010)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Hanson, M.S., et al. Am. J. Physiol. Heart Circ. Physiol. 295 (2), H786-H793 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-179

List Size:
400 ul

List Price:

$495.00

Datasheet
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