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Western blot analysis of PDE8A Antibody (L572) in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A antibody (L572), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PDE8A Antibody (L572) in HL60 cell line lysates (35ug/lane)

PDE8A Antibody

CATALOG NUMBER: 57-875

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDE8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557-587 amino acids from the Central region of human PDE8A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDE8A
Additional Names:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A, PDE8A
Protein Accession Number:
O60658
Ncbi Gene Id Number:
5151
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDE8A plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase, which has a high affinity for cAMP, may be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
Background Reference 1:
Wu,P., Proc. Natl. Acad. Sci. U.S.A. 101 (51), 17634-17639 (2004)
Background Reference 2:
Gamanuma,M., Cell. Signal. 15 (6), 565-574 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-875

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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