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Antibody is used in Western blot to detect PDGFA in HL60 cell lysate.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Antibody is used in Western blot to detect PDGFA in HL60 cell lysate.

PDGFA Antibody

CATALOG NUMBER: 59-024

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDGFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human PDGFA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDGFA
Additional Names:
Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF1
Protein Accession Number:
P04085
Ncbi Gene Id Number:
5154
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDGFA is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.
Background Reference 1:
Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003). Gianni, D., et al., J. Biol. Chem. 278(11):9290-9297 (2003). Muller, C., et al., J. Biol. Chem. 278(20):18330-18335 (2003). Chui, C.M., et al., Cytokine 21(2):51-64 (2003). Laprise, M.H., et al., Blood 100(10):3578-3587 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-024

List Size:
400 ul

List Price:

$495.00

Datasheet
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