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Antibody staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:1000 dilution + human platelet lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of PDGFRA using rabbit polyclonal PDGFRA Antibody (Y720) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Antibody staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

PDGFRA Antibody

CATALOG NUMBER: 63-117

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Chicken, Xenopus
Immunogen:
This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
123 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDGFRA
Additional Names:
Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2, CD140a, PDGFRA, PDGFR2, RHEPDGFRA
Protein Accession Number:
P16234
Protein Gi Number:
129892
Ncbi Gene Id Number:
5156
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Background Reference 1:
Wilczynski, S.P., et al., Hum. Pathol. 36(3):242-249 (2005).
Background Reference 2:
Hiwatari, M., et al., Leukemia 19(3):476-477 (2005).
Background Reference 3:
Debiec-Rychter, M., et al., Gastroenterology 128(2):270-279 (2005).
Background Reference 4:
Sakurai, S., et al., Hum. Pathol. 35(10):1223-1230 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-117

List Size:
400 ul

List Price:

$495.00

Datasheet
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