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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-PDGFRB Pab in Y79 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of PDGFRB Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

PDGFRB Antibody

CATALOG NUMBER: 63-122

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDGFRB antibody is generated from rabbits immunized with human recombinant PDGFRB protein.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
124 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDGFRB
Additional Names:
Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140b, PDGFRB, PDGFR, PDGFR1
Protein Accession Number:
P09619
Protein Gi Number:
129890
Ncbi Gene Id Number:
5159
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides.
Background Reference 1:
Wu, H., et al., J. Biol. Chem. 278(42):40425-40428 (2003).
Background Reference 2:
Whiteman, E.L., et al., Endocrinology 144(9):3811-3820 (2003).
Background Reference 3:
Uren, A., et al., Oncogene 22(15):2334-2342 (2003).
Background Reference 4:
Steer, E.J., et al., Acta Haematol. 107(2):113-122 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-122

List Size:
400 ul

List Price:

$495.00

Datasheet
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