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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
PDHA2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

PDHA2 Antibody

CATALOG NUMBER: 55-394

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDHA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the Central region of human PDHA2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDHA2
Additional Names:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial, PDHE1-A type II, PDHA2, PDHAL
Protein Accession Number:
P29803
Ncbi Gene Id Number:
5161
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Background Reference 1:
Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-394

List Size:
400 ul

List Price:

$495.00

Datasheet
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