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Western blot analysis of extracts of various cell lines, using PDIA2 antibody (14-628) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody (14-628) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PDIA2 antibody (14-628) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PDIA2 Antibody

CATALOG NUMBER: 14-628

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
Mouse liver
Positive Control 3:
Rat liver
Predicted Molecular Weight:
Observed: 58kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PDIA2
Additional Names:
protein disulfide-isomerase A2, protein disulfide isomerase-associated 2, protein disulfide isomerase, pancreatic, protein disulfide isomerase A2, pancreatic protein disulfide isomerase, pancreas-specific protein disulfide isomerase, Rho GDP dissociation inhibitor gamma, OTTHUMP00000197166, OTTHUMP00000067350, PDIR, PDIP, PDI, PDA2, LA16c-314G4.2, protein disulfide isomerase family A, member 2, PDIA2
Ncbi Gene Id Number:
64714
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-628

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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