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45-108 staining (0.1ug/ml) of Daudi lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-108 staining (0.1ug/ml) of Daudi lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

PDIA3 Antibody

CATALOG NUMBER: 45-108

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-EEKPKKKKKAQEDL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in lysates of cell lines NIH3T3, HepG2 and Daudi (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was for 1 hour.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDIA3
Additional Names:
PDIA3, GRP58, ERp57, protein disulfide isomerase family A, member 3, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58 kDa microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58kDa, phospholipase C-alpha, protein disulfide isomerase-associated 3, ER60, protein disulfide-isomerase A3
Protein Accession Number:
NP_005304.3
Protein Gi Number:
21361657
Ncbi Gene Id Number:
2923
Background Reference 1:
Koivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI. Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21. Genomics. 1997 Jun 15;42(3):397-404.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-108

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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