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46-154 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-154 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-154 (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-154 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PDIp Antibody

CATALOG NUMBER: 46-154

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-NLETTKKYAPVD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Pancreas lysates (calculated MW of 58.2kDa according to NP_006840.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine and Heart. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDIA2
Additional Names:
PDIA2, PDIP, protein disulfide isomerase family A, member 2 , LA16c-314G4.2, PDA2, PDI, PDIR , Rho GDP dissociation inhibitor gamma, pancreatic protein disulfide isomerase, protein disulfide isomerase, pancreatic, protein disulfide isomerase-associated 2
Protein Accession Number:
NP_006840.2
Protein Gi Number:
94966757
Ncbi Gene Id Number:
64714
Background Reference 1:
Uehara T, Nakamura T, Yao D, Shi ZQ, Gu Z, Ma Y, Masliah E, Nomura Y, Lipton SA. S-nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration. Nature. 2006 May 25;441(7092):513-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-154

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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