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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Antibody used in WB on Human Jurkat 1 ug/ml.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

PDK1 Antibody

CATALOG NUMBER: 25-028

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PDK1 antibody can be used for detection of PDK1 by ELISA at 1:1562500. PDK1 antibody can be used for detection of PDK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PDK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PDK1
Additional Names:
PDK1, PDHK1
Protein Accession Number:
NP_002601
Protein Gi Number:
4505689
Ncbi Gene Id Number:
5163
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Ohba, T., (2008) Biochem. Biophys. Res. Commun. 369 (2), 376-381.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-028

List Size:
100 ul

List Price:

$519.00

Datasheet
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