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Western blot analysis in RD cell line and mouse heart,rat heart lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in RD cell line and mouse heart,rat heart lysates (35ug/lane).

PDK2 Antibody

CATALOG NUMBER: 64-135

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDK2 antibody is generated from rabbits immunized with recombinant protein of human PDK2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDK2
Additional Names:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2
Protein Accession Number:
Q15119
Protein Gi Number:
21431820
Ncbi Gene Id Number:
5164
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Background Reference 1:
Li, J., et al. J. Biol. Chem. 284(49):34458-34467(2009) Fencl, F., et al. Pediatr. Nephrol. 24(5):983-989(2009) Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009) Hiromasa, Y., et al. Biochemistry 47(8):2312-2324(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-135

List Size:
400 ul

List Price:

$519.00

Datasheet
Shipping Info

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