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Antibody used in IHC on Human Muscle.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in WB on Human Huh7, HepG2 at: 1:50.
Antibody used in IHC on Human Muscle.

PDK4 Antibody

CATALOG NUMBER: 27-707

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PDK4 antibody can be used for detection of PDK4 by ELISA at 1:312500. PDK4 antibody can be used for detection of PDK4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
46 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PDK4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PDK4
Additional Names:
PDK4, FLJ40832, PDHK4
Protein Accession Number:
NP_002603
Protein Gi Number:
4505693
Ncbi Gene Id Number:
5166
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Background Reference 1:
Kimura, K., (2006) Genome Res. 16 (1), 55-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-707

List Size:
100 ul

List Price:

$519.00

Datasheet
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