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Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml
Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [7C1] (RF16024) <br> Blue: DAPI staining
Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [7C1] (RF16024) <br> Blue: DAPI staining
Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.
Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.
Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.
Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml
Trial Size

PDL2 Antibody [7C1]

CATALOG NUMBER: RF16024

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
PD-L2 antibody was raised against the extracellular domain of human PD-L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Application Note:
PD-L2 antibody can be used for detection of PD-L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 38 kDa
Purification:
PD-L2 Antibody is supplied as protein A purified IgG2b.
Clonality:
Monoclonal
Isotype:
IgG2b
physical-state:
Liquid
Buffer:
PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
PD-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDCD1LG2
Additional Names:
PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2
Protein Accession Number:
NP_079515
Protein Gi Number:
190014605
Ncbi Gene Id Number:
80380
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
Background Reference 1:
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Background Reference 2:
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
Background Reference 3:
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Background Reference 4:
Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

RF16024

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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