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46-157 staining (0.1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
46-157 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-157 staining (0.1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

PDLIM1 Antibody

CATALOG NUMBER: 46-157

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TPPEGYEVVTVFPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Human Lung lysate (calculated MW of 36.1kDa according to NP_066272.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDLIM1
Additional Names:
PDLIM1, CLIM1, CLP36, ELFIN, CLP-36, hCLIM1, PDZ and LIM domain 1 (elfin), carboxy terminal LIM domain protein 1, PDZ and LIM domain 1, elfin, enigma
Protein Accession Number:
NP_066272.1
Protein Gi Number:
13994151
Ncbi Gene Id Number:
9124
Background Reference 1:
Kotaka M, Kostin S, Ngai S, Chan K, Lau Y, Lee SM, Li H, Ng EK, Schaper J, Tsui SK, Fung K, Lee C, Waye MM. Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2. J Cell Biochem. 2000 Jun 12;78(4):558-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-157

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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