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Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: L6 whole cell lysate Lysates/proteins at 20 ug per lane.
PDP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat,HepG2,293 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: L6 whole cell lysate Lysates/proteins at 20 ug per lane.

PDP1 Antibody

CATALOG NUMBER: 56-435

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
This PDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDP1
Additional Names:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, PDP 1, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, PDP1, PDP, PPM2C
Protein Accession Number:
Q9P0J1
Protein Gi Number:
78099789
Ncbi Gene Id Number:
54704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pyruvate dehydrogenase (E1) is one of the three components(E1, E2, and E3) of the large pyruvate dehydrogenase complex.Pyruvate dehydrogenase kinases catalyze phosphorylation of serineresidues of E1 to inactivate the E1 component and inhibit thecomplex. Pyruvate dehydrogenase phosphatases catalyze thedephosphorylation and activation of the E1 component to reverse theeffects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenasephosphatase is a heterodimer consisting of catalytic and regulatorysubunits. Two catalytic subunits have been reported; one ispredominantly expressed in skeletal muscle and another one is ismuch more abundant in the liver. The catalytic subunit, encoded bythis gene, is the former, and belongs to the protein phosphatase 2C(PP2C) superfamily. Along with the pyruvate dehydrogenase complexand pyruvate dehydrogenase kinases, this enzyme is located in themitochondrial matrix. Mutation in this gene causes pyruvatedehydrogenase phosphatase deficiency. Multiple alternativelyspliced transcript variants encoding different isoforms have beenidentified.
Background Reference 1:
Kato, J., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 3), 342-345 (2010) :
Background Reference 2:
Cameron, J.M., et al. Hum. Genet. 125(3):319-326(2009)
Background Reference 3:
Stellingwerff, T., et al. Am. J. Physiol. Endocrinol. Metab. 290 (2), E380-E388 (2006) :
Background Reference 4:
Maj, M.C., et al. J. Clin. Endocrinol. Metab. 90(7):4101-4107(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-435

List Size:
400 ul

List Price:

$495.00

Datasheet
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