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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

PDPK1 Antibody

CATALOG NUMBER: 27-307

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDPK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PDPK1 antibody can be used for detection of PDPK1 by ELISA at 1:312500. PDPK1 antibody can be used for detection of PDPK1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
63 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PDPK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PDPK1
Additional Names:
PDPK1, MGC20087, MGC35290, PDK1, PRO0461, PkB-like, PkB-like 1, PDPK2
Protein Accession Number:
NP_002604
Protein Gi Number:
4505695
Ncbi Gene Id Number:
5170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.
Background Reference 1:
Lin, H.J., (2005) Br. J. Cancer 93 (12), 1372-1381.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-307

List Size:
100 ul

List Price:

$519.00

Datasheet
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