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42-765 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-765 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PDPN Antibody

CATALOG NUMBER: 42-765

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-EKVDGDTQTTVEKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Experiments gave bands at approx 25kDa and 37kDa in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. The 37kDa band corresponds to earlier findings in literature with different antibodies (Boucherot et al, Nephrol Dial Transplant. 2002 Jun;17(6):978-84; PMID: 12032185). This protein has a calculated MW of 24.9kDa according to NP_006465.3. Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PDPN
Additional Names:
PDPN, podoplanin, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, OTTHUMP00000009640, OTTHUMP00000044504, glycoprotein, 36-KD, hT1alpha-1, hT1alpha-2, lung type I cell membrane associated glycoprotein, lung type-I cell membrane-associated glycoprotein, lung type-I cell membrane-associated glycoprotein (T1A-2)
Protein Accession Number:
NP_006465.3,NP_938203.2,NP_001006625.1,NP_001006626.1
Protein Gi Number:
54792056
Ncbi Gene Id Number:
10630

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-765

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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