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Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.

PDX1 Antibody

CATALOG NUMBER: 60-968

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-459 amino acids from the C-terminal region of human PDX1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PDHX
Additional Names:
Pyruvate dehydrogenase protein X component, mitochondrial, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, proX, PDHX, PDX1
Protein Accession Number:
O00330
Ncbi Gene Id Number:
8050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Background Reference 1:
Ling, M., et al., Hum. Mol. Genet. 7(3):501-505 (1998). Harris, R.A., et al., J. Biol. Chem. 272(32):19746-19751 (1997). Murray, J., et al., FEBS Lett. 529 (2-3), 173-178 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-968

List Size:
400 ul

List Price:

$495.00

Datasheet
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