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Western blot analysis of extracts of various cell lines, using PDYN antibody (19-886) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using PDYN antibody (19-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PDYN antibody (19-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PDYN antibody (19-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PDYN antibody (19-886) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PDYN antibody (19-886) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PDYN Antibody

CATALOG NUMBER: 19-886

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-254 of human PDYN (NP_077722.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
Mouse liver
Positive Control 3:
Mouse pancreas
Predicted Molecular Weight:
Observed: 28kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PDYN
Additional Names:
ADCA, PENKB, SCA23, proenkephalin-B, beta-neoendorphin-dynorphin, leu-enkephalin, leumorphin, neoendorphin-dynorphin-enkephalin prepropeptide, preprodynorphin, preproenkephalin B, rimorphin
Ncbi Gene Id Number:
5173
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-886

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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