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Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody. The lane on the right is treated with the antigen-specific peptide.

PEA-15 (phospho Ser104) Antibody

CATALOG NUMBER: 79-799

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PEA-15 (phospho Ser104) antibody was raised against a peptide sequence around phosphorylation site of Serine 104 (I-P-S (p)-A-K) derived from Human PEA-15.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.
Predicted Molecular Weight:
19 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PEA15
Additional Names:
PE15, PEA15, PED
Protein Accession Number:
NP_003759.1
Protein Gi Number:
32470612
Ncbi Gene Id Number:
8682
Background:
Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Background Reference 1:
Estelles A., J. Biol. Chem. 271:14800-14806(1996).
Background Reference 2:
Condorelli G., EMBO J. 17:3858-3866(1998).
Background Reference 3:
Wolford J.K., Gene 241:143-148(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-799

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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