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42-236 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-236 (5ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
42-236 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PEBP4 Antibody

CATALOG NUMBER: 42-236

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RERASEPKHKNQAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Heart lysates (calculated MW of 25.7kDa according to NP_659399.2). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PEBP4
Additional Names:
PEBP4, phosphatidylethanolamine-binding protein 4, CORK-1, CORK1, GWTM1933, MGC22776, PRO4408, cousin-of-RKIP 1 protein
Protein Accession Number:
NP_659399.2
Protein Gi Number:
116812622
Ncbi Gene Id Number:
157310
Background Reference 1:
Trynka G, Zhernakova A, Romanos J, Franke L, Hunt K, Turner G, Platteel M, Ryan AW, de Kovel C, Barisani D, Bardella MT, McManus R, Van Heel DA, Wijmenga C. Coeliac disease associated risk variants in TNFAIP3 and REL implicate altered NF-{kappa}B signalling. Gut. 2009 Feb 24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-236

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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