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Western blot analysis of PECI Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PECI using rabbit polyclonal PECI Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.
Western blot analysis of PECI Antibody in HepG2 cell line lysates (35ug/lane)

PECI Antibody

CATALOG NUMBER: 63-592

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECI2
Additional Names:
Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3),delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Protein Accession Number:
O75521
Ncbi Gene Id Number:
10455
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.
Background Reference 1:
Scanlan,M.J., et.al., Int. J. Cancer 83 (4), 456-464 (1999)
Background Reference 2:
Suk,K., et.al., Biochim. Biophys. Acta 1454 (1), 126-131 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-592

List Size:
400 ul

List Price:

$495.00

Datasheet
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