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The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.
The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.

Perforin Antibody [CE2.10]

CATALOG NUMBER: XA-1009

Clonality:
Monoclonal
Tested Applications:
IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Perforin (monoclonal CE2.10) antibody was raised against recombinant mouse perforin (aa. 98-534).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
Perforin antibody can be used for detection of horse Perforin by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apoPerforin. Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Purification:
Perforin (monoclonal CE2.10) antibody was purified to ≥95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG2a
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
Perforin antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
5551
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Perforin and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997).
Background Reference 2:
Perforin: can't live with it-can't live without it:J.C. Reed; Cell 91, 559 (1997).
Background Reference 3:
Prevention of apoptosis by Bcl-2: release of Perforin from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997).
Background Reference 4:
Apaf-1, a human protein homologous to C. elegans CED-4, participates in Perforin-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1009

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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