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46-167 (0.01ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-167 (2ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-167 (0.01ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Perilipin Antibody

CATALOG NUMBER: 46-167

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PREKPKRRVSDS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2ug/ml.This product is not suitable for IF but the related product EB07728 is tested in this application.
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PLIN
Additional Names:
PLIN, perilipin, PERI, lipid droplet-associated protein
Protein Accession Number:
NP_002657.2
Protein Gi Number:
54112125
Ncbi Gene Id Number:
5346
Background Reference 1:
Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 2006 Oct 9;580(23):5484-91. Epub 2006 Sep 1. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-167

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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