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43-544 (0.1ug/ml) staining of Rat Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-544 (0.1ug/ml) staining of Rat Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Peripherin 1 Antibody

CATALOG NUMBER: 43-544

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-QKEQHSDLDKSSIH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Rat Colon lysates (calculated MW of 54.0kDa according to NP_036765.2). Recommended concentration: 0.1-0.3ug/ml. An additional faint band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1).
Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Peripherin 1
Additional Names:
Prph, peripherin, Prph1, peripherin 1
Protein Accession Number:
NP_038667.2,NP_001157060.1,NP_001157061.1
Protein Gi Number:
254675335
Ncbi Gene Id Number:
19132
Background Reference 1:
McLean JR, Robertson J.Isoform-specific expression and ratio changes accompany oxidant-induced peripherin aggregation in a neuroblastoma cell line.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-544

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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