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Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of Periphilin in human colon tissue with Periphilin antibody at 2.5 μg/mL.
Immunofluorescence of Periphilin in human colon tissue with Periphilin antibody at 5 μg/mL.
Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

Periphilin Antibody

CATALOG NUMBER: 6687

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Periphilin antibody was raised against a 16 amino acid synthetic peptide near the center of human Periphilin.
The immunogen is located within amino acids 160 - 210 of Periphilin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Periphilin antibody can be used for detection of Periphilin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Purification:
Periphilin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Periphilin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Periphilin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPHLN1
Additional Names:
Periphilin Antibody: HSPC206, HSPC232, Periphilin-1, Gastric cancer antigen Ga50
Protein Accession Number:
NP_958848
Protein Gi Number:
48255929
Ncbi Gene Id Number:
51535
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Periphilin Antibody: Periphilin, known as PPHLN1 or gastric cancer antigen Ga50, plays a role in epithelial differentiation and contributes to epidermal integrity and barrier formation. It interacts with periplakin, a known precursor of the cornified cell envelope. Periphilin 1 is ubiquitously expressed and localizes to nucleus and cytoplasm. Existing as eight alternatively spliced isoforms, Periphilin is highly insoluble and contains a putative nuclear localization signal (NLS) within its N-terminal half, a prerequisite for the formation of insoluble complexes and a possible caspase recognition sequence and a potential nuclear export signal. Periphilin may play an important role in the nervous system.
Background Reference 1:
Kazerounian S and Aho S. Characterization of periphilin, a widespread, highly insoluble nuclear protein and potential constituent of the keratinocyte cornified envelope. J. Biol. Chem. 2003; 278:36707-17.
Background Reference 2:
Kurita M, Suzuki H, Masai H, et al. Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. Biochem. Biophys. Res. Commun. 2004; 324:554-61
Background Reference 3:
Soehn AS, Pham TT, Schaeferhoff K, et al. Periphilin is strongly expressed in the murine nervous system and is indispensable for murine development. Genesis 2009; 47:697-707
Background Reference 4:
Soehn AS, Franck T, Biskup S, et al. Periphilin is a novel interactor of synphilin-1, a protein implicated in Parkinson's disease. Neurogenetics 2010; 11:203-15

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6687

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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