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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PERP 2451 (1 μg/mL), PERP, 57-777 (2 μg/mL), beta-actin 3779 (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PERP 2451 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PERP 2451 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunocytochemistry Validation of PERP in A431 Cells</strong><br>Immunocytochemical analysis of A431 cells using anti-PEPR antibody (2451) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: PERP 2451 (1 μg/mL), PERP, 57-777 (2 μg/mL), beta-actin 3779 (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

PERP Antibody

CATALOG NUMBER: 2451

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat (100%); Mouse (82%)
Immunogen:
Anti-PERP antibody (2451) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP.
The immunogen is located within the last 50 amino acids of PERP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
WB: 1-2 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 21kD
Observed: 21 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar PERP expression profile in human cell lines detected by two independent anti-PERP antibodies that recognize different epitopes, 2451 against C-terminus domain and 57-777 against C-terminus domain.  PERP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human PERP has one isoform (193aa, 21kD). Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD). 2451 can detect human, mouse and rat.
Purification:
PERP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PERP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PERP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Perp
Additional Names:
PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik, Krtcap1, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, KCP-1
Protein Accession Number:
NP_071404
Protein Gi Number:
11528520
Ncbi Gene Id Number:
64058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Background Reference 1:
Matsuoka et al. Science 1998; 282:1893-7.
Background Reference 2:
Tanaka et al. Nature 2000; 404:42-9.
Background Reference 3:
Oda et al. Science 2000; 288:1053-8.
Background Reference 4:
Oda et al. Cell 2000; 102:849-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2451

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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