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To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Persephin (38-119) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPersephin.
To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Persephin (38-119) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPersephin.

Persephin Antibody (biotin)

CATALOG NUMBER: 38-120

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPersephin. Human Persephin specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hPersephin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Persephin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.
Western Blot:
To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Persephin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PSPN
Additional Names:
PSPPersephinPSP
Protein Accession Number:
O60542
Protein Gi Number:
7531178
Ncbi Gene Id Number:
5623
User Note:
Centrifuge vial prior to opening.
Background:
Persephin is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. Persephin has a similar structure to other family members, glial cell line-derived neurotrophic factor, Artemin and neurturin. All these proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-120

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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