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<strong>Figure 1 WB Validation in Human and Mouse Cell Lines</strong><br>Loading: 15 μg of lysate Antibodies: PEX10 9671, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 WB Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane.Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 WB Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PEX10 9671, 4 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen </strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PEX10 in Mouse Thymus</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PEX10 in Rat Testis</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with 9671 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysate Antibodies: PEX10 9671, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

PEX10 (CT) Antibody

CATALOG NUMBER: 9671

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), horse (100%), gorilla (100%), chimpanzee (100%)
Immunogen:
Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10.

The immunogen is located within last 50 amino acids of PEX10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 4 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 – A549 Cell Lysate
Positive Control 2:
Cat. No. 17-003 – A549 Cell Slide
Predicted Molecular Weight:
Predicted: 37kD

Observed: 37 kD
Isoforms:
Human PEX10 has 2 isoforms, including isoform 1 (326aa, 37.1kD) and isoform 2 (346aa, 39.2kD). Mouse PEX10 has 1 isoform (324aa, 37.2kD). 9671 can detect PEX10 in human and mouse.
Purification:
PEX10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PEX10 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PEX10
Additional Names:
PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69
Protein Accession Number:
O60683
Protein Gi Number:
3914299
Ncbi Gene Id Number:
5192
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PEX10 is implicated in the biogenesis of peroxisomes. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome.
Background Reference 1:
Warren D.S. et al. Am. J. Hum. Genet. 63:347-359(1998)
Background Reference 2:
Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
Background Reference 3:
Ota T. et al. Nat. Genet. 36:40-45(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9671

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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