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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br>Loading: 15 μg of lysate Antibodies: PEX3 9665, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane.Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PEX3 9665, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of PEX3 in Human Spleen</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PEX3 in Mouse Thymus</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PEX3 in Rat testis</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with 9665 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in Human Cell Lines
Loading: 15 μg of lysate Antibodies: PEX3 9665, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

PEX3 (CT) Antibody

CATALOG NUMBER: 9665

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), cow (100%)
Immunogen:
Anti-PEX3 antibody (9665) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 – A549 Cell Lysate
Positive Control 2:
Cat. No. 17-003 – A549 Cell Slide
Predicted Molecular Weight:
Predicted: 42kD

Observed: 42 kD
Isoforms:
Human PEX3 has 1 isoform, including isoform 1 (373aa, 42kD). Mouse PEX3 has 1 isoform (372aa, 42kD). Rat PEX3 has one isoform (372aa, 42kD). 9665 can detect PEX3 in human, mouse and rat.
Purification:
PEX3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PEX3 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PEX3
Additional Names:
PEX3 Antibody: Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3
Protein Accession Number:
P56589
Protein Gi Number:
3914303
Ncbi Gene Id Number:
8504
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PEX3 Antibody: PEX3 is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, PEX3 is necessary for the import of peroxisomal membrane proteins in the peroxisomes. Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. Mutations in the PEX19-binding domain of PEX3 reduce the affinity for PEX19 and destabilize PEX3.
Background Reference 1:
Ghaedi et al. Mol Biol Cell. 2000; 11(6):2085-2102
Background Reference 2:
Fang et al. J Cell Biol. 2004;164(6):863-875
Background Reference 3:
Hattula et al. PloS One. 2014; 9(7):e103101
Background Reference 4:
Schmidt et al. Traffic. 2012; 13(9):1244-1260

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9665

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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