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To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human PF-4 (XP-5256) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPF-4.
To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human PF-4 (XP-5256) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPF-4.

PF-4 Antibody (biotin)

CATALOG NUMBER: XP-5256Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human PF-4 (XP-5256) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
Western Blot:
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 μg/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hPF-4 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
PF-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PF4
Additional Names:
PF-4, CXCL4, SCYB4, Platelet factor 4, C-X-C motif chemokine 4, PF-4
Protein Accession Number:
P02776
Protein Gi Number:
130304
Ncbi Gene Id Number:
5196
User Note:
Centrifuge vial prior to opening.
Background:
Platelet factor 4 (PF-4) is a 70 amino acid protein that is released from the alpha granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF-4 probably has a role in inflammation and wound repair.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5256Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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