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Western blot analysis of extracts of various cell lines, using PFDN4 antibody (15-973) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using PFDN4 antibody (15-973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PFDN4 antibody (15-973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PFDN4 antibody (15-973) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PFDN4 antibody (15-973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PFDN4 Antibody

CATALOG NUMBER: 15-973

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human PFDN4 (NP_002614.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
DU145
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
MCF7
Positive Control 5:
mouse kidney
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PFDN4
Additional Names:
C1, PFD4, prefoldin subunit 4, prefoldin 4, protein C-1
Ncbi Gene Id Number:
5203
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-973

List Size:
100 uL

List Price:

$436.00

Datasheet
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