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Western blot analysis of extracts of various cell lines, using PFKP antibody (23-278) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and PFKP knockout (KO) HeLa cells, using PFKP antibody (23-278) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using PFKP antibody (23-278) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PFKP antibody (23-278) (40x lens).
Immunofluorescence analysis of U-2 OS cells using PFKP antibody (23-278) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PFKP antibody (23-278) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
KO

PFKP Antibody, KO Validated

CATALOG NUMBER: 23-278

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human PFKP
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF-7
Positive Control 2:
SKOV3
Positive Control 3:
HeLa
Positive Control 4:
NCI-H460
Positive Control 5:
Mouse brain
Positive Control 6:
Rat heart
Predicted Molecular Weight:
Observed: 100kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PFKP
Additional Names:
ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255, HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255, HAMAP-Rule:MF_03184}, PFK-P, 27111 {ECO:0000255, HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255, HAMAP-Rule:MF_03184}, PFKP, PFKF
Ncbi Gene Id Number:
5214
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-278

List Size:
100 uL

List Price:

$436.00

Datasheet
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