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Western blot analysis in WiDr cell line lysates (35ug/lane).
PGA4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in WiDr cell line lysates (35ug/lane).

PGA4 Antibody

CATALOG NUMBER: 56-802

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This PGA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-381 amino acids from the C-terminal region of human PGA4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PGA4
Additional Names:
Pepsin A-4, Pepsinogen-4, PGA4
Protein Accession Number:
P0DJD7
Protein Gi Number:
378521995
Ncbi Gene Id Number:
643847
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PGA4 shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Background Reference 1:
Fujinaga, M., et al. Protein Sci. 4(5):960-972(1995)
Background Reference 2:
Athauda, S.B., et al. J. Biochem. 106(5):920-927(1989)
Background Reference 3:
Evers, M.P., et al. Genomics 4(3):232-239(1989)
Background Reference 4:
Foltmann, B. FEBS Lett. 241 (1-2), 69-72 (1988) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-802

List Size:
400 ul

List Price:

$495.00

Datasheet
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