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Western blot analysis of extracts of various cell lines, using PGAM1 antibody (19-247) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using PGAM1 antibody (19-247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

PGAM1 Antibody

CATALOG NUMBER: 19-247

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
LO2
Positive Control 3:
A375
Positive Control 4:
MCF7
Positive Control 5:
NIH/3T3
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PGAM1
Additional Names:
PGAM1, phosphoglycerate mutase 1 (brain), PGAMA, RP11-452K12.8, OTTHUMP00000059414, Phosphoglycerate mutase A, nonmuscle form, PGAM2, phosphoglycerate mutase 2 (muscle), MGC88743, PGAMM, Phosphoglycerate mutase, muscle form, PGAM3, PGAM4, phosphoglycerate mutase family member 4, PGAM-B, dJ1000K24.1, phosphoglycerate mutase family 3, phosphoglycerate mutase family 4, phosphoglycerate mutase processed protein, CDABP0006
Ncbi Gene Id Number:
5223
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-247

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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