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46-171 (0.05ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig Liver (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-171 (0.05ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig Liver (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PGAM1 Antibody

CATALOG NUMBER: 46-171

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KAMEAVAAQGKAKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 28.8kDa according to NP_002620.1; NP_000281.2 and of NP_001025062.1). The additional 30kDa band observed in the rodent lysates likely represent the acetylated form. Recommended concentration: 0.05-0.15ug/ml.
Specificity:
Please note this antibody is expected to recognize the products of 3 highly similar genes.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PGAM1 / PGAM2 / PGAM4
Additional Names:
PGAM1, PGAM2, phosphoglycerate mutase 1 (brain), PGAMA, RP11-452K12.8, OTTHUMP00000059414, Phosphoglycerate mutase A, nonmuscle form, phosphoglycerate mutase 2 (muscle), MGC88743, PGAMM, Phosphoglycerate mutase, muscle form, PGAM3, phosphoglycerate mutase family member 4, PGAM-B, dJ1000K24.1, phosphoglycerate mutase family 3, phosphoglycerate mutase family 4, phosphoglycerate mutase processed protein, bisphosphoglycerate mutase 1, bisphosphoglycerate mutase 2, bisphosphoglycerate mutase 4, BPG-dependent PGAM 1, OTTHUMP00000020190, PGAM-M, BPG-dependent PGAM 2, OTTHUMP00000063546
Protein Accession Number:
NP_002620.1,NP_000281.2,NP_001025062.1
Protein Gi Number:
4505753
Ncbi Gene Id Number:
5223;5224;441531
Background Reference 1:
Sakoda S, Shanske S, DiMauro S, Schon EA. Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family. J Biol Chem. 1988 Nov 15;263(32):16899-905.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-171

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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