Western blot analysis of various lysates using PGE receptor EP2 (PTGER2) Rabbit mAb at 1:1000 dilution incubated overnight at 4°C.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3 s.

Immunohistochemistry analysis of paraffin-embedded Human placenta using PGE receptor EP2 (PTGER2) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using PGE receptor EP2 (PTGER2) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using PGE receptor EP2 (PTGER2) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PGE receptor EP2 (PTGER2) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using PGE receptor EP2 (PTGER2) Rabbit mAb at 1:1000 dilution incubated overnight at 4°C. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3 s.

PGE receptor EP2 (PTGER2) Rabbit Recombinant Antibody (84-286)

CATALOG NUMBER: 84-286

Clonality:

Monoclonal

Tested Applications:

ELISA, IF/ICC, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Tested Applications:

ELISA, IF/ICC, IHC-P, WB

Application Note:

WB,1:1000 - 1:6000, IHC-P,1:100 - 1:1000, IF/ICC,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

HeLa,A-549,THP-1,Mouse lung,Mouse liver,Mouse spleen,Rat testis

Predicted Molecular Weight:

40kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

PTGER2

Additional Names:

EP2; PGE receptor EP2 (PTGER2)

Protein Accession Number:

P43116

Ncbi Gene Id Number:

5732

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

84-286

List Size:

100 uL

List Price:

$477.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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PGE receptor EP2 (PTGER2) Rabbit Recombinant Antibody (84-286)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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