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Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) at 1:1000 at each lane.

PGK1 Antibody

CATALOG NUMBER: 62-700

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PGK1
Additional Names:
Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Protein Accession Number:
P00558
Protein Gi Number:
52788229
Ncbi Gene Id Number:
5230
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
Background Reference 1:
Shetty, S., et al., Am. J. Respir. Cell Mol. Biol. 31(1):100-106 (2004).
Background Reference 2:
Daly, E.B., et al., Biochim. Biophys. Acta 1691(1):17-22 (2004).
Background Reference 3:
Daly, E.B., et al., Int. J. Biol. Markers 19(2):170-172 (2004).
Background Reference 4:
Saito, Y., et al., Biochem. Biophys. Res. Commun. 314(2):396-402 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-700

List Size:
400 ul

List Price:

$519.00

Datasheet
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