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Western blot analysis of PGLS Antibody in Ramos cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PGLS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PGLS Antibody in Ramos cell line lysates (35ug/lane)

PGLS Antibody

CATALOG NUMBER: 61-611

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PGLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-220 amino acids from the C-terminal region of human PGLS.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PGLS
Additional Names:
6-phosphogluconolactonase, 6PGL, PGLS
Protein Accession Number:
O95336
Ncbi Gene Id Number:
25796
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PGLS hydrolyzes 6-phosphogluconolactone to 6-phosphogluconate.
Background Reference 1:
Miclet,E., et.al., J. Biol. Chem. 276 (37), 34840-34846 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-611

List Size:
400 ul

List Price:

$495.00

Datasheet
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