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Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 μg/mL.
Immunohistochemistry of PHAP in human small intestine tissue with PHAP antibody at 10 μg/mL.
Immunofluorescence of PHAP in human small intestine tissue with PHAP antibody at 20 μg/mL.
Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 μg/mL.
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PHAP Antibody

CATALOG NUMBER: 3152

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Immunogen:
PHAP antibody was raised with a synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.
The immunogen is located within the last 50 amino acids of PHAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PHAP antibody can be used for detection of PHAP I and PHAP III by Western blot at 1 μg/mL. At approximately 35 kDa and 32 kDa a band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 3:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
35 kDa
Purification:
PHAP Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PHAP Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHAP antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANP32A
Additional Names:
PHAP Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32
Protein Accession Number:
P39687
Protein Gi Number:
730318
Ncbi Gene Id Number:
8125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHAP Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Background Reference 1:
Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6.
Background Reference 2:
Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3152

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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