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Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 μg/mL.
Immunohistochemistry of PHAP in human small intestine tissue with PHAP antibody at 10 μg/mL.
Immunofluorescence of PHAP in human small intestine tissue with PHAP antibody at 20 μg/mL.
Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 μg/mL.
Trial Size

PHAP Antibody

CATALOG NUMBER: 3152

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Immunogen:
PHAP antibody was raised with a synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.
The immunogen is located within the last 50 amino acids of PHAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PHAP antibody can be used for detection of PHAP I and PHAP III by Western blot at 1 μg/mL. At approximately 35 kDa and 32 kDa a band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 3:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
35 kDa
Purification:
PHAP Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANP32A
Additional Names:
PHAP Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32
Protein Accession Number:
P39687
Protein Gi Number:
730318
Ncbi Gene Id Number:
8125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHAP Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Background Reference 1:
Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6.
Background Reference 2:
Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3152

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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