Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Western Blot Validation in (A) Human Raji Cells , (B) Mouse testis tissue lysate and (C) Rat testis tissue lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PHAP I 3151 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PHAP I 3145 (2 μg/mL), PHAP I 3151 (1 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human, Mouse and Rat Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PHAP I 3151 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: PHAP I 3151 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of PHAP I in Mouse Small Intestine cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Small Intestine Cells labeling PHAP I with 3151 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 6 Immunohistochemistry Validation of PHAP I in Mouse Small Intestine Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded Mouse Small Intestine Tissue using anti-PHAP I antibody (3151) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 KD Validation of PHAPI in Human Breast Cancer Cells (Schafer et al., 2006) </strong><br>Human breast cancer cells (T47D cells) were transfected with control or PHAPI siRNA duplex. PHAPI was detected via Western Blot analysis by using the anti-PHAPI antibody. PHAPI expression was reduced after PHAPI siRNA knockdown.
<strong>Figure 8 Increased Expression Validation of PHAPI in Patient Samples of BreastTumor Tissue (Schafer et al., 2006) </strong><br> PHAPI was overexpressed in all breast tumor samples of patients and human breast cancer cells (MDA-MB-453), but not in the normal breast tissue or human primary mammary epithelial cells (HMEC).
<strong>Figure 9 Overexpression of PHAPI in Breast Cancer Cells (Schafer et al., 2006) </strong><br>Western blot analysis with anti-PHAPI antibodies was performed for PHAPI in human cell lines from breast, prostate and lung. PHAPI was overexpressed in breast cancer cells when compared with normal cells (HMEC) whereas there were no significant differences in PHAPI expression in normal and cancer cells of either prostate or lung origin.
Figure 1 Western Blot Validation in (A) Human Raji Cells , (B) Mouse testis tissue lysate and (C) Rat testis tissue lysate
Loading: 15 μg of lysates per lane.Antibodies: PHAP I 3151 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

PHAP I Antibody

CATALOG NUMBER: 3151

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Immunogen:
Anti-PHAP I antibody (3151) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PHAP I .
The immunogen is located within the last 50 amino acids of PHAP I.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 1 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Positive Control 3:
Cat. No. 1476 - Rat Testis Tissue Lysate
Positive Control 4:
Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Predicted Molecular Weight:
Predicted: 29kD
Observed: 29 kD
Purification:
PHAP I Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHAP I Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHAP I antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANP32A
Additional Names:
PHAP I Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32
Protein Accession Number:
P39687
Protein Gi Number:
730318
Ncbi Gene Id Number:
8125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Background Reference 1:
Jiang et al. Science. 2003;299(5604):223-6.
Background Reference 2:
Nicholson and Thornberry. Science. 2003 10;299(5604):214-5.
Citation 1:
Schafer et al. Enhanced sensitivity to cytochrome c-induced apoptosis mediated by PHAPI in breast cancer cells. Cancer Res. 2006;66(4):2210-8. PMID: 16489023
Citation 2:
Kurokawa et al. A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53. Sci Signal. 2013;6(274):ra32. PMID: 23652204
Citation 3:
Parrish. Regulation of Apoptosis Following Mitochondrial Cytochrome c Release. Department of Pharmacology and Molecular Cancer Biology, Duke University. PhD Thesis, 2010.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3151

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369